dc.contributor.author | Düzgün, Özer Özad | |
dc.contributor.author | Okumuş, Funda | |
dc.contributor.author | Saral, Ayşegül | |
dc.contributor.author | Çopur Çiçek, Ayşegül | |
dc.contributor.author | Cinemre, Sedanur | |
dc.date.accessioned | 2020-10-28T07:17:16Z | |
dc.date.available | 2020-10-28T07:17:16Z | |
dc.date.issued | 2019 | en_US |
dc.identifier.citation | Düzgün, A. Ö., Okumuş, F., Saral, A., Çiçek, A. Ç., & Cinemre, S. (2019). Determination of antibiotic resistance genes and virulence factors in Escherichia coli isolated from Turkish patients with urinary tract infection. Revista da Sociedade Brasileira de Medicina Tropical, 52. Doi: 10.1590/0037-8682-0499-2018 | en_US |
dc.identifier.uri | https://hdl.handle.net/11494/2390 | |
dc.description.abstract | Introduction: Escherichia coli ranks among the most common sources of urinary tract infections (UTI). Methods: Between
November 2015 and August 2016, 90 isolates of E. coli were isolated from patients at Rize Education and Research Hospital
in Turkey. Antibiotic susceptibility was determined for all isolates using the Kirby–Bauer disk diffusion method. These E. coli
isolates were also screened for virulence genes, β-lactamase coding genes, quinolone resistance genes, and class 1 integrons by
PCR. Results: With respect to the antibiotic resistance profile, imipenem and meropenem were effective against 98% and 90%
of isolates, respectively. A high percentage of the isolates showed resistance against β lactam/β lactamase inhibitor combinations,
quinolones, and cephalosporins. PCR results revealed that 63% (57/90) of the strains carried class 1 integrons. In addition, a
high predominance of extended-spectrum β-lactamases (ESBLs) was observed. The qnrA, qnrB, and qnrS genes were found in
24 (26.6%), 6 (6.6%), and 3 (3.3%), isolates, respectively. The most common virulence gene was fim (82.2%).The afa, hly, and
cnf1 genes were detected in 16.6%, 16.6%, and 3.3% of isolates, respectively. Moreover, we observed eleven different virulence
patterns in the 90 E. coli isolates. The most prevalent pattern was fım, while hly-fım, afa-aer-cnf-fım, aer-cnf, afa-aer, and afacnf-fım patterns were less common. Conclusions: Most of the E. coli virulence genes investigated in this study were observed
in E. coli isolates from UTI patients. Virulence genes are very important for the establishment and maintenance of infection. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Soc Brasileira Medicina Tropical | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Quinolones | en_US |
dc.subject | Virulence genes | en_US |
dc.subject | UTI | en_US |
dc.title | Determination of antibiotic resistance genes and virulence factors in Escherichia coli isolated from Turkish patients with urinary tract infection | en_US |
dc.type | article | en_US |
dc.relation.journal | Revista da Sociedade Brasileira de Medicina Tropical | en_US |
dc.department | AÇÜ, Sağlık Bilimleri Fakültesi | en_US |
dc.authorid | 0000-0002-6301-611X | en_US |
dc.authorid | 0000-0002-7757-6812 | en_US |
dc.identifier.volume | 52 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1590/0037-8682-0499-2018 | en_US |
dc.contributor.institutionauthor | Saral, Ayşegül | en_US |