Effects of cold stratification and H2SO4 pre-treatments on germination of sea buckthorn (Hippophae Rhamnoides L.) seeds under open field and greenhouse conditions
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CitationÖlmez, Z., & Olcum, B. A. (2017). Effects of cold stratification and H2SO4 pre-treatments on germination of sea buckthorn (HippophaeRhamnoides L.) seeds under open field and greenhouse conditions. Fresenius Environmental Bulletin, 26(8), 5142-5146.
This study was carried out to determine the effects of some pre-treatments including soaking in concentrate (96%) H2SO4 for 1, 2, 3 and 4 minutes and cold stratification for 10, 20, 30, 40 and 50 days on seed germination and to investigate how to overcome dormancy of Hippophae rhamnoides L. seeds. The seeds were sown in the greenhouse and under open field conditions. The statistical approach was a randomized complete block design with three replications. Germinated seeds were observed periodically during 35 days to determine germination percentages and germination rates. The highest germination percentages (44.4% and 75.5%) were obtained from the seeds soaked in H2SO4 for 2 minute and sown both open field conditions and in the greenhouse, respectively. The lowest germination percentages were obtained from the control seeds which were sown under both two conditions (20.7% and 39.2%). While the best germination rate (11 days) was determined from 3-minutes H2SO4 pre treatments in the greenhouse, 10-day cold stratification gave the best germination rate (9 days) under open field conditions.