Isolation, characterization and pathogenicity of fungi from pristiphora abietina (hymenoptera: tenthredinidae)
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Citationİskender, N. A., Örtücü, S., Aksu, Y., & Saral, A. (2017). Isolation, characterization and pathogenicity of fungi from Pristiphora abietina (Hymenoptera: Tenthredinidae). Fresenius Environmental Bulletin, 26(1-A), 628-633.
This study was conducted to isolate and characterize pathogenic fungi for possible use in biocontrol of P. abietina and to determine their pathogenicity. Eleven fungal isolates were obtained from larvae. Based on traditional and molecular methods, fungal isolates were identified as Beauveria bassiana, Penicillium corylophilum, P. glabrum, P. polonicum, Aspergillus niger, A. versicolor, Cladosporium sphaerospermum, Alternaria sp. and Trichoderma asperellum. At first, all these fungal isolates were tested against P. abietina larvae for isolate selection at 106 conidia/ml and 100% relative humidity (RH). Three fungal isolates ((PaF 004, PaF 009, PaF 076) belonging to Beauveria genus were determined to be pathogen against P. abietina. Then, mortality of these three isolates, on P. abietina larvae were investigated at three conidial concentrations as 10(6), 10(7) and 10(8), and at the degree of humidity as in the 65 5% and 100% RH, respectively. In 100% RH and at 10(7)-10(8) conidia/ml, three fungal isolates have shown a mortality rate of 100%. On the other hand, in 65 5% RH and at 10(7)-10(8) conidia/ml, three fungal isolates have shown a mortality rate between 49.1 +/- 4.52% - 91.1 +/- 7.2% and 63,3 +/- 4,5 - 95,3 +/- 2,7, respectively (p<0.05). The present results indicated that PaF 009 has an excellent potential for biological control of P. abietina larvae. This is also the first report on the larvacidal effect of B. bassiana on P. abietina.