The in vitro antioxidative properties of the essential oils and methanol extracts of Satureja spicigera (K. Koch.) Boiss. and Satureja cuneifolia ten
Akpulat, Hüseyin Aşkın
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This study was dcsigııed to examine the in vitro antioxidant activities of the essential oil and methanol extracts of Satııreja Jpicigera and S. cımeifo/ia from Turkish flora. GC and GC/MS analysis of the essential oils resulted in the identification of 40 and 29 compounds, representing the 99.4% and 99.5% of the oils, respectively. Major constituents of tlıe oils werc carvacrol (42.5% and 67.1%), y-terpinene (21.5'Yo and 15.2%) and p-cymcne (20.9% and 6.7%), respectively. Mctlıanol extracts were alsa obtaincd froın the aerial parts of the plants. The saınples were subjccted to a screening for their possible antioxidant activitics by using 2,2-diphcnyl - l-picrylhydrazyl (DPPH) and [l-carotcnc-linoleic acid assays. ln general, samplcs obtained from S.·cııııeifolia exerted greater antioxidant activities than did those obtained froın S. spicigera . In the DPPH test system, free radical-scavenging activity of S. spicigera oil was detennined to be 127 ± 1 .63 rıg/ml, whcrcas 1C50 value of S. cwıeifo/ia was 89. 1 ± 2.29 rıg/ml. in the p-carotene-linoleic acid test system, antioxidant activities of the oil wcrc 81.7 ± 1.14% ancl 93.7 ± J .83%, respectively. Antioxiclant activities of the synthetic antioxiclant, BHT, ascorbic acid, curcumin and cı.-tocopherol were also determinecl in parallel experiments. © 2005 Elsevier Ltcl. All rights reservccl.