The effect of hesperidin and quercetin on oxidative stress, NF-kappa B and SIRT1 levels in a STZ-induced experimental diabetes model
Citation
Iskender, H., Dokumacıoğlu, E., Şen, T. M., İnce, I., Kanbay, Y., & Saral, S. (2017). The effect of hesperidin and quercetin on oxidative stress, NF-κB and SIRT1 levels in a STZ-induced experimental diabetes model. Biomedicine & Pharmacotherapy, 90, 500-508. Doi: 10.1016/j.biopha.2017.03.102Abstract
Objective: The aim of this study is to investigate the roles of SIRT1 and NF-kB in the pathogenesis of
diabetes mellitus in rats with STZ-induced diabetes and determine the effects of hesperidin and
quercetin on oxidative stress and on the levels of SIRT1 and NF-kB.
Materials and methods: The experimental animals were divided into four groups, each group comprising
ten rats designated as follows: group 1 served as control rats (C); group 2 served as diabetic rats (DM);
group 3 served as diabetic rats administered hesperidin (DM + HSP) (100 mg/kg b.w.) in aqueous
suspension orally for 15 days; and group 4 served as diabetic rats administered quercetin (DM + Q)
(100 mg/kg b.w.) in aqueous suspension orally for 15 days.
Results: In diabetic group, liver and kidney SIRT1, SOD and CAT activities were significantly lower than
control group (p < 0.05). Hesperidin and quercetin caused significant increase in the SIRT1, SOD and CAT
activities of both DM + HP and DM + Q groups kidney tissues compared to DM group (p < 0.05). Liver SOD
activies were not found to differ significantly between DM, DM + Q and DM + HP groups (p > 0.05). In
DM + HP group, liver CAT activities were significantly higher than DM (p < 0.05), but there was no
significant difference in liver CAT activities between DM and DM + Q (p > 0.05). In diabetic group, liver and
kidney NF-kB and MDA levels were increased compared to control group (p < 0.05), and groups of
DM + HP and DM + Q had lower NF-kB and MDA levels than diabetic group (p < 0.05).
Conclusion: As a conclusion, based on the results we obtained from this study and the literature data
discussed above, we determined in STZ-induced diabetic rats that, increased glucose levels and liver and
kidney damage markers decreased significantly after administration of hesperedin and quercetin, and
that oxidative stress and NF-kB levels increased while SIRT1 levels decreased in the diabetic group