Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities
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2017Author
Erenler, RamazanMeral, Bilal
Şen, Özkan
Elmastaş, Mahfuz
Aydın, Ali
Eminağaoğlu, Özgür
Topçu, Gulacti
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Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55(1), 1646-1653.Abstract
Context: Origanum (Lamiaceae) has been used in food and pharmaceutical industries.
Objective: Isolation and identification of bioactive compounds from Origanum rotundifolium Boiss. and
investigation of their antiproliferative and antioxidant activities.
Materials and methods: The aerial part of O. rotundifolium was dried and powdered (1.0 kg ±2.0 g) then
extracted with hexane, ethyl acetate, methanol and water. Solvent (3 1 L) was used for each extraction
for a week at room temperature. The aqueous extract was partitioned with ethyl acetate (3 1 L) to yield
the water/EtOAc extract subjected to chromatography to isolate the active compounds. The structures of
isolated compounds were elucidated by 1 D, 2 D NMR and LC-TOF/MS.
Results: Apigenin (1), ferulic acid (2), vitexin (3), caprolactam (4), rosmarinic acid (5), and globoidnan A
(6) were isolated and identified. Globoidnan A (6), vitexin (3), and rosmarinic acid (5) revealed the excellent DPPH scavenging effect with IC50 values of 22.4, 31.4, 47.2 lM, respectively. Vitexin (3) (IC50 3.6), globoidnan A (6) (IC50 4.6), apigenin (1) (IC50 8.9) and ferulic acid (2) exhibited more ABTSþ activity than
standard Trolox (IC50 13.8 lg/mL). Vitexin (3) revealed the most antiproliferative activity against HeLa,
HT29, C6 and Vero cells lines with IC50 values of 35.6, 32.5, 41.6, 46.7 (lM), respectively.
Discussion and conclusion: Globoidnan A (6) has the most antioxidant effects on all assays. This has to
do with the chemical structure of the compound bearing the acidic protons. Vitexin (3) could be a promising anticancer agent.