Application of proline to root medium is more effective for amelioration of photosynthetic damages as compared to foliar spraying or seed soaking in maize seedlings under short-term drought
Künye
Demiralay, M., Altuntaş, C., Sezgin, A., Terzi, R., & Kadıoğlu, A. (2017). Application of proline to root medium is more effective for amelioration of photosynthetic damages as compared to foliar spraying or seed soaking in maize seedlings under short-term drought. Turkish Journal of Biology, 41(4), 649-660.Özet
Exogenous proline (PRO) at low concentrations can enhance drought stress tolerance in different application modes such as
application to rooting medium, foliar spray, and seed soaking. However, there is no information about which application mode is more
effective for increasing the drought tolerance. Comparative effects of 1, 10, and 20 mM PRO applications through three application
modes to hydroponically grown seedlings were examined under short-term drought stress in maize seedlings. Effects on leaf water
potential, membrane damage, chlorophyll content, proline level, and gas exchange parameters such as net photosynthetic rate (Pn),
transpiration rate (E), stomatal conductance (gs), and substomatal CO2
concentration (Ci) were compared. Results indicated that PRO
pretreatments raised the water potential, chlorophyll content, Pn, E, gs, and Ci but lowered the malondialdehyde content in the three
application modes as compared to the untreated plants. Of the three different modes of PRO pretreatment, rooting medium treatment at
1 mM concentration was also more effective in alleviating stress-induced damages in maize seedlings. Moreover, effectively applied PRO
increased the maximum quantum efficiency of PS II, quantum yield of PS II photochemistry, photochemical quenching, and electron
transport rate but decreased nonphotochemical quenching of chlorophyll fluorescence under short-term drought stress. In conclusion,
exogenous PRO was markedly more effective in the root-treated mode than in foliar spray or seed soaking mode, suggesting that PRO
had a different ameliorating effect in different application modes. Proline application in an effective mode can induce photochemical
efficiency under short-term drought in maize.