Investigation of class 1 integrons with antibiotic resistance genes in multidrug-resistant acinetobacter baumannii strains and determination of plant extract effects on multidrug-resistant isolates
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2021Author
Saral, AyşegülCinemre, Sedanur
Okumuş, Funda
Akar, Zeynep
Çiçek, Ayşegül Çopur
Düzgün, Azer Özad
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Saral, A., Cinemre, S., Okumuş, F., Akar, Z., Çopur Çiçek, A., & Düzgün, A. (2021). Investigation of class 1 integrons with antibiotic resistance genes in multidrug-resistant acinetobacter baumannii strains and determination of plant extract effects on multidrug-resistant isolates, Farmacia, 69(5), 914-918.Abstract
This study aimed to investigate the presence of resistance genes in multidrug-resistant A. baumannii isolates as well as to determine the antibacterial activity of selected plant extracts against isolates. 41 strains were isolated from various clinical samples. PCR tests were performed using the primers. Methanol was used as solvent for the preparation of the plant extracts. MIC values of the plant extracts were determined by the broth microdilution method. The bla(OXA-)(23), bla(CTX-M-1), bla(CTX-M-2), bla(GES) genes and Class 1 integrons were detected in five isolated strains. The lowest MIC value (2.25 mg/mL) was determined for the Echinacea putpurea extract, while the highest MIC value (50 mg/mL) was determined for the Morus alba extract. Determination of the antibacterial effect of plants extracts used in the study against A. baumannii isolates shows the importance of screening the antibacterial activity of plants in the fight against antibiotic resistance. Studiul a avut ca scop investigarea genelor de rezistență ale izolatelor de A. baumannii multirezistente, precum și
determinarea activității antibacteriene a unor extracte vegetale supra a 41 specii microbiene izolate din diferite probe clinice.
Metanolul a fost folosit ca solvent de extracție. Valorile concentrației minime inhibitorii ale extractelor obținute au fost
determinate prin metoda microdiluției. Genele blaOXA-23, blaCTX-M-1
, blaCTX-M2 și blaGES, alături de integroni din clasa 1 au
fost decelate pentru 5 specii izolate. Valoarea MIC cea mai scăzută (2,25 mg/mL) a fost obținută pentru extractul din
Echinaceea purpurea, în timp ce cea mai mare valoare MIC (50 mg/mL) a fost determinată pentru extractul de Morus alba.
Determinarea efectului antibacterian ale extractelor vegetale împotriva izolatelor de A. baumannii arată importanța screeningului activității antibacteriene a plantelor în lupta împotriva rezistenței la antibiotice.