Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey

Iraz, MeryemDüzgün, Azer ÖzadÇopur Çiçek, AyşegülBonnin, Remy A.Ceylan, AyşenurSaral, AyşegülNordmann, PatriceSandallı, Cemal2020-11-132020-11-132014Iraz, M., Düzgün, A. O., Çiçek, A. C., Bonnin, R. A., Ceylan, A., Saral, A., ... & Sandallı, C. (2014). Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in Turkey. Diagnostic Microbiology and Infectious Disease, 78(3), 292-294.https://hdl.handle.net/11494/2456Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = I). PCR and DNA sequence analysis revealed that 1 strain possessed the bla(GES-5) and another carried a novel bla(VIM) variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. (C) 2014 Elsevier Inc. All rights reserved.eninfo:eu-repo/semantics/closedAccessPseudomonas aeruginosaCarbapenemaseβ-lactamaseESBLClass 1 integronCharacterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing β-lactamases in Pseudomonas aeruginosa in TurkeyCharacterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing beta-lactamases in Pseudomonas aeruginosa in TurkeyArticle78329229410.1016/j.diagmicrobio.2013.12.003Q2N/A