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dc.contributor.authorDanışmazğlu, Mehtap
dc.contributor.authorNalçacıoğlu, Kadriye
dc.contributor.authorMuratoğlu, Hacer
dc.contributor.authorDemirbağ, Zihni
dc.date.accessioned2020-11-03T08:18:44Z
dc.date.available2020-11-03T08:18:44Z
dc.date.issued2018en_US
dc.identifier.citationDanışmazoğlu, M., Nalcacıoğlu, R., Muratoğlu, H., & Demirbağ, Z. (2018). The protein-protein interactions between Amsacta moorei entomopoxvirus (AMEV) protein kinases (PKs) and all viral proteins. Virus research, 248, 31-38. Doi: 10.1016/j.virusres.2018.02.007en_US
dc.identifier.urihttps://hdl.handle.net/11494/2401
dc.description.abstractEntomopoxviruses are an important group of viruses infecting only insects. They belong to Poxviridae which infect both invertebrates and vertebrates, including humans. Protein kinases are known to have roles at virus morphogenesis, host selectivity, the regulation of cell division and apoptosis in some vertebrate poxviruses. In this study, 2 protein kinases (PKs) (AMV153 and AMV197) of Amsacta moorei entomopoxvirus (AMEV) were investigated for the interactions among 230 viral proteins using yeast two-hybrid system (Y2H). For this purpose, two protein kinases and 230 viral genes were cloned into the bait and prey vectors, respectively. Bait vectors were introduced into Saccharomyces cerevisiae AH109. Expression of the bait genes were confirmed by western blot analysis. Both yeast strains of bait were transformed individually with each prey clone and grown on a selective medium (minimal synthetic defined) to determine the protein–protein interactions between bait and prey proteins. Transformations identified totally 16 interactions among AMEV protein kinases and all viral proteins of which 5 belong to AMV153 and 11 belong to AMV197. One of the five interactions detected for AMV153 protein kinase is self-association. Its other four interactions are with two virus entry complex proteins (AMV035 and AMV083), a membrane protein (AMV165) and a subunit of RNA polymerase (AMV230). The other protein kinase, AMV197, interacted with two virus entry complex proteins (AMV035 and AMV083) as AMV153, a caspase-2 enzyme (AMV063), a Holliday junction resolvase (AMV162), a membrane protein (AMV165), a subunit of RNA polymerase (AMV230) and five other hypothetical proteins (AMV026, AMV040, AMV062, AMV069, AMV120) encoded by AMEV genome. Glutathione S-transferase (GST) pull-down assay was used to confirm all interactions described by Y2H analysis. In addition, the theoretical structures of the two of 16 interactions were interpreted by docking analysis. Consistent with Y2H and pull down assays, docking analysis also showed the interactions of AMV063 with AMV153 and AMV197. Detected interactions of the AMEV viral proteins with viral protein kinases could lead to the understanding of the regulation of the viral activities of interacted viral proteins.en_US
dc.language.isoengen_US
dc.publisherElsevier Science BVen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmsacta moorei entomopoxvirusen_US
dc.subjectSerine/threonine protein kinasesen_US
dc.subjectYeast two-hybrid systemen_US
dc.subjectProtein-protein interactionsen_US
dc.subjectAMV153en_US
dc.subjectAMV197en_US
dc.titleThe protein-protein interactions between Amsacta moorei entomopoxvirus (AMEV) protein kinases (PKs) and all viral proteinsen_US
dc.typearticleen_US
dc.relation.journalVirus Researchen_US
dc.departmentAÇÜ, Sağlık Hizmetlerien_US
dc.authoridTR122862en_US
dc.identifier.volume248en_US
dc.identifier.startpage31en_US
dc.identifier.endpage38en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak113Z081
dc.identifier.doi10.1016/j.virusres.2018.02.007en_US
dc.contributor.institutionauthorDanışmazğlu, Mehtapen_US


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