Isolation, characterization and pathogenicity of bacteria from oligonychus ununguis (jacobi) (acari: tetranychidae)
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CitationIskender, N. A., Aksu, Y., (2016). Isolation, characterization and pathogenicity of bacteria from Oligonychus ununguis (Jacobi)(Acari: Tetranychidae. Fresenius Environmental Bulletin, 25(10), 4163-4170.
This study was conducted to isolate and characterize pathogenic bacteria for possible use in the biocontrol of Spruce Spider Mite Oligonychus ununguis and to determine their pathogenicity. O. ununguis were collected from Savsat Forests, in Artvin and transported to the laboratory to be used in the experiments. According to the results of our study; eight bacteria species belonging to seven genera of O. ununguis were isolated. Some morphological,biochemical and other phenotypic properties of bacterial isolates were determined by routine and conventional methods (API 20E, API 50 CH and API Staph kits). In addition, 16S rRNA gene region also was sequenced. Based on studies, bacterial isolates were identified as Methylobacterium sp. (Ou1), Bacillus thuringiensis (Ou2), Staphylococcus chromogenes (Ou3), Pseudomonas oleovorans (Ou4), Streptomyces sp. (Ou6), Paenibacillus apiarius (Ou7), Mycobacterium sp. (Ou8) and Bacillus cereus (Ou9). All these bacteria were tested against adults of O. ununguis within ten days. Bacillus thuringiensis (Ou2) was found to be causing the highest pathogenicity (40.7%).