In vitro and in silico evaluation of some plant extracts and phytocompounds against multidrug-resistant Gram-negative bacteria
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2021Author
Aydemir, EdaSarıyer, Emrah
Akyıldız, Esma
Özad Düzgün, Azer
Camadan, Yasemin
Saral Sarıyer, Ayşegül
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Aydemir, E., Sarıyer, E., Akyıldız, E., Özad Düzgün, A., Camadan, Y., & Saral Sarıyer, A. (2021). In vitro and in silico evaluation of some plant extracts and phytocompounds against multidrug-resistant Gram-negative bacteria. Advances in Traditional Medicine, 1-11.Abstract
The spread of multidrug-resistant Gram-negative (MDR) bacteria is a global public health problem, as infections caused by
MDR Gram-negative bacteria are difcult to treat. New antibiotic agents need to be developed to overcome this problem,
and phytochemicals show promise at this point. In this study, methanol extracts were prepared from cinnamon, thyme,
nettle, white tea, rosehip, and antibacterial activity of the methanol extracts was studied against two MDR Gram-Negative
bacteria (K. pneumoniae and A. baumannii) by broth microdilution method. The MICs of methanol extracts of cinnamon,
rosehip, thyme, white tea for A. baumannii were found as 0.015125 g/ml, 0.07825 g/ml, 0.030625 g/ml, 0.00796875 g/ml,
respectively. It was found that only cinnamon methanol extract had antibacterial activity in the used extract concentrations
against K. pneumoniae and the MIC value was 0.0605 g/ml. The efects of plant methanol extract with antibacterial activity
and imipenem combinations were studied in vitro using the checkerboard method. The FIC Indexes were obtained from the
checkerboard results and it was observed that the combination of methanol extract and imipenem showed an antagonistic
or additive/indiferent efect but not a synergistic efect. We evaluated the binding afnity of epigallocatechin 3-gallate,
quercetin, cinnamaldehyde, carvacrol, and thymol phytocompounds using in silico methods, which are well known as a
phytocompounds in white tea, cinnamon, thyme, nettle, and rosehip and have antibacterial activities. The results suggested
that these phytocompounds should be supported with in vivo and in vitro experiments to investigate their potential for being
inhibitor candidates.