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    Chemical compositions and larvicidal effectiveness on nerium oleander flower essential oil on hyphantria cunea larvae
    (Gheorghe Asachi Technical University of Iasi, Romania, 2024) Göktürk, Temel; Dumbadze, Guguli; Jgenti, Lali
    In this research, the chemical composition of oleander (Nerium oleander L) (Apocynaceae) flower essential oil was analyzed using GC-MS (Gas Chromatography-Mass Spectrometer), and its larvicidal effectiveness on five Fall webworm (Hyphantria cunea Drury) larval stages was investigated. The essential oil was extracted from the flowers of N. oleander by the hydrodistillation method using the Clevenger apparatus. A total of 48 chemical constituents present in oleander essential oil were identified using Gas Chromatography-Mass Spectrometry analysis. Camphore (10.3%), Digitoxigenine (10.21%), Eugenol (8.61%), Thymol (6.15%), and 1.8-Cineole (5.05%) were found as the primary components of oil. It is observed that the applications of N. oleander essential oil at doses of 10, 15, and 20 μL/Petri and the controls at 24, 48, 72, and 96 hours induced fatalities at varied rates (40.4-100%) on five H. cunea larval stages (L1-L5). The highest toxicity was identified at L1 (0.56 μL/larva), and the lowest toxicity was determined at L5 (12.64 μL/larva) according to LD50 and LD90 values. The research was conducted in laboratory settings during 2021-22. In conclusion, N. oleander essential oil showed high toxicity against L1 and L2 larval stages of H. cunea.
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    Insecticidal effects of some essential oils against box tree moth (Cydalima perspectalisWalker (Lepidoptera: Crambidae))
    (Springer, 2020) Göktürk, Temel; Chachkhiani Anasashvili, Nunu; Kordalı, Şaban; Dumbadze, Guguli; Bozhüyük, Ayşe Usanmaz
    The box tree mothCydalima perspectalis(Walker) (Lepidoptera: Crambidae) is one of the most alien insects found in theBuxusareas of Georgia and World. Many methods have been used to control this pest up to now. But, the problem is still going on. In this study, insecticidal effects of the essential oils obtained from plantsArtemisia absinthiumL.,Seriphidium santonicum(L.) Sojak, Seriphidium spicigerum(K.Koch) Poljakov,Cuminum cyminumL.,Mentha pulegiumL., Origanum majoranaL.,Origanum onitesL., Origanum syriacumL.,Origanum vulgareL., andSatureja hortensisL. onC.perspectaliswere tested in laboratory conditions. In this context, larvae of the 2nd and 5th instars ofC.perspectaliswere exposed to essential oils at doses of 10, 15 and 20 mu l/petri for 24, 48, 72 and 96 h. All of the essential oils used in the study caused mortality at different rates; the highest effect on 2nd and 5th instar larvae ofC.perspectaliswas obtained with the essential oil fromO.oniteswith a mortality rate of 80.0-71.6%. The oils fromO. onites(73.3-65.0%), O.syriacum(73.3-63.3%),O.majorana(71.6-66.6%),A.absinthium(68.3-61.6%),S.santonicum(68.3-60.0%),S.spicigerum(66.6-60.0%),S.hortensis(66.6-61.1%),C.cyminum(58.3-53.3%) andM.pulegium(51.6-45.0%) followed this in this order. As a results of the dose effect tests conducted in the second part of the study, the most toxic plant essential oils were determined to be fromO.vulgareand the lowest toxic effect fromM.pulegiumbased on LD(50)and LD90. The results obtained show that the essential oils fromO.vulgarecan be used in the control againstC.perspectalis
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    Natural insecticides: Effectiveness of essential oils against Orasanga Japonica in nymphal and adult stages
    (Springer Nature, 2025) Göktürk, Temel; Dumbadze, Guguli; Jgenti, Lali
    Orosanga japonica Melichar (Hemiptera: Ricaniidae) is an invasive polyphagous pest in the eastern Black Sea coastal zone of Turkey. Synthetic broad-spectrum insecticides are often used to control O. japonica. The increased risk of chemical contamination when using synthetic insecticides to control insect pests has led to a growing interest in essential oils. In this study, the insecticidal effect of the essential oils of Artemisia absinthium, Cuminum cyminum, Mentha pulegium, Rhododendron ponticum and Rhododendron luteum on the nymphal and adult stages of O. japonica was tested under laboratory conditions. 10, 15 and 20 µL/Petri essential oils were sprayed on adults and nymphs of O. japonica. It was observed that mortality rates increased with increasing dose and exposure time in all experiments. The oils caused a mortality rate of 6.7–80.8% in the nymphs and 0.8–55.8% in the adults at 20 µL/Petri after 96 h of exposure. It was found that the essential oils of R. ponticum and R. luteum had the strongest insecticidal effect on the nymphal and adult stages of O. japonica. The highest mortality occurred at a dose of 20 µL/Petri of the oils after 96 h of exposure. The positive control had the highest mortality rate for nymphs and adults (100%). R. ponticum and R. luteum proved to be the most toxic essential plant oils in the second part of the study. C. cyminum, on the other hand, had the least toxic effect, based on LD50 and LD90.