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    HPLC method for the analysis of chlorogenic acid of Viburnum tinus L. and Viburnum orientale Pallas
    (Turkish Pharmacists Association, 2015) Özbilgin, Serkan; Ergene, Burçin; Altun, Mehmet Levent; Sever Yilmaz, Betül; Saltan, Gülçin; Yüksel, Emrah
    A simple and sensitive method for separation and determination of chlorogenic acid has been developed. Chlorogenic acid was separated using a Supelcosil LC 18 column (250 x 4.6 mm, 5 μm) by gradient elution at the flow rate of 1.2 mL/min. The composition of mobile phase consisted of ophosphoric acid, bidistilled water (0.2%) and acetonitrile. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for chlorogenic acid was between 0.7237-500 μg/mL. The method described was suitable for the determination of chlorogenic acid in the leaves, branches and fruits of Viburnum tinus L. and Viburnum orientale Pallas. It was observed that V.orientale fruit sample has the highest chlorogenic acid content (0.5069%), while V.tinus branches (0.0141%) have the lowest chlorogenic acid content as w/w (%) in our case. This study is the first application of High Performance Liquid Chromatography (HPLC) method to the determination of chlorogenic acid content of V. tinus and V. orientale in Turkey.
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    Neuroprotective potential of Viburnum orientale Pallas through enzyme inhibition and antioxidant activity assays
    (Elsevier Science BV, 2018) Orhan, İlkay Erdoğan; Şenol, Fatma Sezer; Yılmaz, Betül Sever; Altun, Mehmet Levent; Özbilgin, Serkan; Yazgan, A. N.; Yüksel, Emrah; İşcan, Gülçin Saltan
    Neurobiological effects of the fruit, leaf, and branch extracts of Viburnum orientale, used in the traditional drink called "gilaburi" in Anatolia, were tested against acetyl-(AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR). Antioxidant effect of the extracts was determined using six in vitro methods. The fruit and branch methanol extracts with the richest total phenolic contents exerted the highest inhibition against all enzymes. These extracts were the most active in DPPH radical scavenging, ferric-(FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) assays, which was followed by the leaf aqueous extract with the best nitric oxide (75.00 +/- 1.22%), DMPD scavenging (33.70 +/- 1.13%), and metal-chelating (54.66 +/- 3.56%) effects at 2500 mu g/mL. We previously reported that the fruits are rich in chlorogenic acid, which might be related to its marked enzyme inhibitory and antioxidant effect. Our results indicated that all methanol and leaf aqueous and branch extracts of the plant are promising for their neuroprotective potential. (c) 2017 SAAB. Published by Elsevier B.V. All rights reserved.