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    Angiotensin II type 1 receptor (AT1) gene A1166C is associated with the risk of hypertension
    (Mary Ann Liebert Inc., 2015) Bayramoğlu, Ayşegül; Kurt, Hülyam; Güneş, Hasan Veysi; Ata, Necmi; Birdane, Alparslan; Dikmen, Miris; Üstüner, Mehmet Cengiz; Çolak, Ertuğrul; Değirmenci, İrfan
    Aim: This study was performed on primary hypertension patients in a Turkish population to determine the frequency of the A1166C polymorphism in the angiotensin II type 1 receptor (AT1) gene and to examine the role of this polymorphism in hypertension development. Materials and Methods: In this study, 250 genomic DNA samples were collected (from 142 hypertension patients and 108 healthy subjects), randomized, and analyzed. Genomic DNA was prepared from peripheral blood using the salt extraction method. The presence of the A1166C polymorphism in the AT1 gene was determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism method. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge-coupled device camera. Results: Genotype distribution and allele frequency A1166C genotype frequency was determined as AA 96.3% and AC 3.7% for controls and as AA 86.6% and AC 13.4% for patients. A statistically significant difference was found between the control group and patients in terms of genotype and allele frequency. Conclusion: Our results suggest that an interaction exists between the AT1 gene polymorphism and hypertension in the Turkish population.
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    Öğe
    Frequency of angiotensin II type 1 receptor gene polymorphism in Turkish acute stroke patients
    (Wiley, 2013) Kurt, Hülyam; Bayramoğlu, Ayşegül; Güneş, Hasan Veysi; Özbabalık, Demet; Değirmenci, İrfan; Çolak, Ertuğrul; Coşan Turgut, Didem; Bayram, Banu; Dikmen, Miris
    This study was performed in acute stroke patients in the Turkish population to determine the frequency of the A1166C polymorphism in the AT1 gene and to examine the role of this polymorphism in acute stroke development. In this study, 257 genomic DNA samples were analysed (from 206 acute stroke patients and 51 healthy individuals). Genomic DNA was prepared from peripheral blood using the salt-extraction method. The presence of the A1166C polymorphism in the AT1 gene was determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge-coupled device (CCD) camera. In this study, the allele frequency at the A1166C position was 92% A and 8% C for control and 97% A and 3% C for patients. This difference in allele frequency between the control group and the patient group was not statistically significant. However, genotype and allele frequencies showed a significant difference (P<0.001) in the control and the patient groups. The results of this study show no relationship between the A1166C polymorphism in the AT1 gene and acute stroke in the Turkish population.