Frequency of angiotensin II type 1 receptor gene polymorphism in Turkish acute stroke patients
Yükleniyor...
Dosyalar
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study was performed in acute stroke patients in the Turkish population to determine the frequency of the A1166C polymorphism in the AT1 gene and to examine the role of this polymorphism in acute stroke development. In this study, 257 genomic DNA samples were analysed (from 206 acute stroke patients and 51 healthy individuals). Genomic DNA was prepared from peripheral blood using the salt-extraction method. The presence of the A1166C polymorphism in the AT1 gene was determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge-coupled device (CCD) camera. In this study, the allele frequency at the A1166C position was 92% A and 8% C for control and 97% A and 3% C for patients. This difference in allele frequency between the control group and the patient group was not statistically significant. However, genotype and allele frequencies showed a significant difference (P<0.001) in the control and the patient groups. The results of this study show no relationship between the A1166C polymorphism in the AT1 gene and acute stroke in the Turkish population.
Açıklama
Anahtar Kelimeler
acute stroke, AT1 gene, A1166C polymorphism
Kaynak
Journal of Cellular and Molecular Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
17
Sayı
4
Künye
Hulyam, K., Ayşegül, B., Veysi, G. H., Demet, O., İrfan, D., Ertuğrul, C., ... & Miris, D. (2013). Frequency of angiotensin II type 1 receptor gene polymorphism in Turkish acute stroke patients. Journal of cellular and molecular medicine, 17(4), 475-481.
