Pistacia terebinthus (Menengiç) Gallerinin Antimikrobiyal etkisi ve GC-MS analizi
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Artvin Çoruh Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pistacia terebinthus galinin antimikrobiyal aktivitesini tespit etmek için disk difüzyon yöntemi kullanıldı. Standart mikroorganizmalarımız (Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922) brain heart infüzyon brothlar içine alınarak Mc Farland 0.5 eşeline uyan konsantrasyonları hazırlandı. Mueller Hinton agar besiyerlerine steril eküvyon çubuklar kullanılarak ekildi. Bakteriler 35.5-36.5 derecede 24 saat inkübe edildi. İnkübasyon sonrası diskler etrafında oluşan inhibisyon bölgeleri bir cetvel yardımıyla ölçülerek değerlendirmeler yapıldı. P. terebinthus bitkisinin gal özütünün GC-MS analizini yapılmıştır. GC spektrumunda yaklaşık 85 bileşen belirlenmiştir, özütün içerdiği bileşenlerin yanısıra ve dekompozisyon ürünleri de mevcuttur. Özüte ait GC kromatogramı verilmiştir, ayrıca analizi yapılabilen fraksiyonlar üzerlerine belirlenen bileşenlerin isimleri ve olasılık oranları belirtilmiştir. P. terebinthus bitkisinin gal özütü gram (+) bakteriler olan Staphylococcus aureus ve Enterococcus faecalis üzerinde olumlu etkiye sahiptir ve büyük ölçüde bakterileri inhibe etmiştir. Antibiyotiklerin zararları düşünüldüğünde çıkardığımız özütün doğal olması ilaç gereksiniminde özütün kullanılmasının daha avantajlı olacağı düşünülmektedir.
Disk diffusion method was used to determine the antimicrobial activity of Pistacia terebinthus gal. Our standard microorganisms (Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922) were taken into brain heart infusion broths and their concentrations were prepared according to Mc Farland 0.5 chart. They were inoculated on Mueller Hinton agar media using sterile swab sticks. Bacteria were incubated at 35.5-36.5 degrees for 24 hours. The inhibition zones formed around the discs after incubation were measured with the help of a ruler and evaluations were made. GC-MS analysis of gal extract of P. terebinthus plant was performed. Approximately 85 components have been identified in the GC spectrum, besides the components contained in the extract and decomposition products are also available. The GC chromatogram of the extract is given, as well as the names and probability ratios of the components determined on the fractions that can be analyzed. Gal extract of P. terebinthus plant has positive effect on gram (+) bacteria Staphylococcus aureus and Enterococcus faecalis and inhibited bacteria to a large extent. Considering the harms of antibiotics, it is thought that it would be more advantageous to use the extract in case of drug requirement, since the extract we extract is natural.
Disk diffusion method was used to determine the antimicrobial activity of Pistacia terebinthus gal. Our standard microorganisms (Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922) were taken into brain heart infusion broths and their concentrations were prepared according to Mc Farland 0.5 chart. They were inoculated on Mueller Hinton agar media using sterile swab sticks. Bacteria were incubated at 35.5-36.5 degrees for 24 hours. The inhibition zones formed around the discs after incubation were measured with the help of a ruler and evaluations were made. GC-MS analysis of gal extract of P. terebinthus plant was performed. Approximately 85 components have been identified in the GC spectrum, besides the components contained in the extract and decomposition products are also available. The GC chromatogram of the extract is given, as well as the names and probability ratios of the components determined on the fractions that can be analyzed. Gal extract of P. terebinthus plant has positive effect on gram (+) bacteria Staphylococcus aureus and Enterococcus faecalis and inhibited bacteria to a large extent. Considering the harms of antibiotics, it is thought that it would be more advantageous to use the extract in case of drug requirement, since the extract we extract is natural.
Açıklama
Anahtar Kelimeler
Pistacia Terebinthus, Aktimikrobiyal, GC-MS, Gal
Kaynak
Turkish Journal of Biodiversity
WoS Q Değeri
Scopus Q Değeri
Cilt
4
Sayı
2
Künye
Akpulat, S. , Traş, M. , Şahinkaya, M. S. & Akpulat, H. A. (2021). Pistacia terebinthus (Menengiç) Gallerinin Antimikrobiyal etkisi ve GC-MS analizi . Turkish Journal of Biodiversity , 4 (2) , 98-104 . DOI: 10.38059/biodiversity.1000001
