Phytochemical analysis of solidago virgaurea: Quantitative analysis of bioactive compounds by LC-MS/MS, ascertaining the antioxidant activity and phenolic profile by potentiometric sensors

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dc.authorid0000-0003-1254-1069
dc.authorid0000-0003-0064-0318
dc.contributor.authorYıldız, İlyas
dc.contributor.authorEminağaoğlu, Özgür
dc.date.accessioned2026-07-21T08:07:55Z
dc.date.available2026-07-21T08:07:55Z
dc.date.issued2025
dc.departmentAÇÜ, Orman Fakültesi, Orman Mühendisliği Bölümü
dc.description.abstractSolidago virgaurea L. subsp. virgaurea (SVSV) has long been used in traditional medicine for treating urinary tract disorders, nephrolithiasis, and inflammatory-related conditions. This study aimed to evaluate the antioxidant capacities and phenolic profiles of SVSV extracts. Antioxidant activities were assessed using the FRAP and DPPH assays. Moreover, total phenolic content was determined by Folin-Ciocalteu reagent (FCR). The advanced technique, a potentiometric sensor, was utilized for antioxidant assays and FCR. Phenolic composition in plant extract was determined by LC–MS/MS. Solidago virgaurea methanolic extract exhibited strong antioxidant potential with a FRAP value of 19.07 µmol TE/mg extract and a DPPH radical scavenging activity of 78.8% at 128 ppm. The total phenolic content was found to be 413.82 mg GA/mg extract. Compared to standards, SVSV methanolic extract showed higher DPPH activity (78.80%) than BHT (68.25%), BHA (49.06%), and Trolox (65.25%), and was the same as with gallic acid (78.55%). LC-MS/MS analysis revealed that the major phenolic compounds were trans-cinnamic acid (126.19 µg/g), chlorogenic acid (58.83 µg/g), and vanillin (26.17 µg/g), along with rutin (11.36 µg/g), gentisic acid (11.03 µg/g), and quercetin (2.47 µg/g). These findings demonstrate that SVSV possesses remarkable antioxidant capacity and a diverse phenolic profile, supporting its potential use as a functional food ingredient and natural source of antioxidants.
dc.identifier.doi10.24925/turjaf.v13i11.3409-3414.8407
dc.identifier.endpage3414
dc.identifier.issue11
dc.identifier.startpage3409
dc.identifier.urihttps://hdl.handle.net/11494/6385
dc.identifier.volume13
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorEminağaoğlu, Özgür
dc.institutionauthorid0000-0003-0064-0318
dc.language.isoen
dc.publisherTurkish Science and Technology Publishing (TURSTEP)
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSolidago virgaurea
dc.subjectAntioxidant activity
dc.subjectPhenolic compounds
dc.subjectFRAP
dc.subjectDPPH
dc.titlePhytochemical analysis of solidago virgaurea: Quantitative analysis of bioactive compounds by LC-MS/MS, ascertaining the antioxidant activity and phenolic profile by potentiometric sensors
dc.typeArticle

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