Lactobacillus plantarum and Lactobacillus helveticus modulate SIRT1, Caspase3 and Bcl-2 in the testes of high-fructose-fed rats

Basit Öğe Kaydı
dc.authorid0000-0003-0090-7369en_US
dc.authorid0000-0002-6422-1203en_US
dc.authorid0000-0002-5432-0304en_US
dc.contributor.authorYıldırım, Onur Gökhan
dc.contributor.authorSadi, Gökhan
dc.contributor.authorAkar, Fatma
dc.date.accessioned2021-02-22T07:20:28Z
dc.date.available2021-02-22T07:20:28Z
dc.date.issued2020
dc.departmentAÇÜ, Sağlık Hizmetlerien_US
dc.description.abstractBackground and Aims: The influence of a high-fructose diet and probiotics on the male reproductive system and the testicular apoptotic pathway has been poorly documented. In this study, we aimed to investigate the influence of Lactobacillus plantarum and Lactobacillus helveticus supplementation on apoptotic factors such as sirtuin1, caspase3 and bcl-2 on the testicular tissue of high-fructose-fed rats. Methods: Fructose was given to the rats as a 20% solution in drinking water for 15 weeks. Gene expressions were established by real-time PCR. Protein levels were determined by Western blot analysis. Results: Fructose consumption did not change mRNA expression of SIRT1, but did resulted in a decreased protein level. Dietary fructose reduced bcl-2 mRNA and protein expressions, whereas no changes were observed in the gene and protein expression levels of factor caspase-3. Both Lactobacillus supplementations increased SIRT1 protein expression without changing the mRNA levels in fructose-fed rats. The supplementations with both probiotics produced a significant downregulation on caspase3 mRNA and protein levels. Bcl-2 proetin level increases with both probiotics supplementation while, mRNA level did not show difference in L.plantarum, but increased in L. helveticus supplementation. Conclusion: Treatments with L.plantarum and L.helveticus can reduce testicular apoptosis induced by dietary high-fructose in rats via suppressing caspase3 and promoting sirt1 and bcl-2 protein expressions.
dc.identifier.citationYıldırım, O. G., Sadi, G., & Akar, F. (2020). Lactobacillus plantarum and Lactobacillus helveticus modulate SIRT1, Caspase3 and Bcl-2 in the testes of high-fructose-fed rats. 50 (3), 168-175en_US
dc.identifier.doi10.26650/IstanbulJPharm.2020.0076Published:DEC2020
dc.identifier.endpage175en_US
dc.identifier.issue3en_US
dc.identifier.startpage168en_US
dc.identifier.urihttps://hdl.handle.net/11494/2634
dc.identifier.volume50en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorYıldırım, Onur Gökhan
dc.language.isoenen_US
dc.publisherIstanbul Univen_US
dc.relation.ispartofİstanbul Journal of Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.rightsAttribution-NonCommercial 3.0 United States*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/us/*
dc.subjectDietary fructoseen_US
dc.subjectLactobacillus plantarumen_US
dc.subjectLactobacillus helveticusen_US
dc.subjectApoptosisen_US
dc.subjectTestesen_US
dc.titleLactobacillus plantarum and Lactobacillus helveticus modulate SIRT1, Caspase3 and Bcl-2 in the testes of high-fructose-fed ratsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
onurgokhan_yildirim.pdf
Boyut:
473.77 KB
Biçim:
Adobe Portable Document Format
Açıklama:
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Sağlık Hizmetleri Meslek Yüksekokulu Yayın Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu