Determination of a novel integron-located variant (bla(OXA-320)) of Class D beta-lactamase in Proteus mirabilis
Yükleniyor...
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel bla(OXA) variant exhibiting one amino acid substitution (Asn266Ile) from bla(OXA-1). This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside-modifying enzymes. According to sequence records, the new variant was named as bla(OXA-320). Cassette array and size of integron were found as bla(OXA-320)-aadA1 and 2086bp, respectively. The bla(OXA-320) gene is not transferable according to conjugation experiment. In this study, we report the first identification of bla(OXA-320)-aadA1 gene cassette, a novel variant of Class D -lactamase, in P. mirabilis from Turkey.
Açıklama
This work was partially supported by Recep Tayyip Erdogan University Research Fund Grants BAP-2009.102.03.2 and BAP-2011.102.03.3.
Anahtar Kelimeler
bla(OXA-320) gene, Class D -lactamase, Integron, P, mirabilis
Kaynak
Journal of Basic Microbiology
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
54
Sayı
10
Künye
Çiçek, A. C., Düzgün, A. O., Saral, A., & Sandallı, C. (2014). Determination of a novel integron‐located variant (bla OXA‐320) of Class D β‐lactamase in Proteus mirabilis. Journal of Basic Microbiology, 54(10), 1030-1035.
