Helvella leucomelaena (Pers.) Nannf.’ın antimikrobiyal ve antioksidan etkisi
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Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Artvin Çoruh Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Attribution 3.0 United States
Attribution 3.0 United States
Özet
Mantarlar içerdikleri aktif bileşenler sayesinde besleyici bir gıda olarak tüketilmelerinin yanı sıra tıbbi ve tedavi edici özelliklerinden dolayı uzun yıllardır halk tarafından kullanılmaktadırlar. Mantarların immünolojik ve antikanser özelliklerinin yanı sıra antioksidan, antihipertensif, kolesterol düşürücü, karaciğer koruyucu, antifibriotik, antiinflamasyon, antidiyabetik, antiviral ve antimikrobiyal etkileri olduğu bilinmektedir. Yenilebilir olan mantarlardan Helvella leucomelaena (Pers.) Nannf. Ascomycota’ nın Helvellaceae’ye ait bir mantar türüdür. Bu çalışmada H. leucomelaena’nın metanol, etanol ve hekzan gibi çözücülerden elde edilen ekstraklarının farklı konsantrasyonları kullanılarak antimikrobiyal aktivitesinin belirlenmesi ve metanol çözücüsü kullanılarak elde edilen ekstraktının farklı konsantrasyonlarının antioksidan aktivitesinin belirlenmesi amaçlanmıştır. Antimikrobiyal aktivitesi disk difüzyon metoduna göre tespit edilmiştir. Çalışma için Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Escherichia coli ATCC25322 ve Bacillus megaterium DSM32 gibi bakteriler, fungus olarak Candida albicans FMC17 mikroorganizmaları kullanılmıştır. Antioksidan aktivitesi ise 2,2-difenil-1-pikrilhidrazil (DPPH) yöntemine göre radikal süpürücü kapasite tespit edilmiştir. Sonuçta H. leucomelaena en yüksek inhibisyon zonunu C. albicans’a karşı (16 mm) 750 µg konsantrasyonda etanol ekstresinde gösterdiği tespit edilmiştir. DPPH radikalini süpürücü etkisi ise artan konsantrasyonlara bağlı olarak arttığı gözlemlenmiştir
Mushrooms have been used by the public for many years due to the active containts they contain as well as being consumed as a nutritious food. It is known that mushrooms have antioxidant, antihypertensive, cholesterol-lowering, liver-protective, antifibriotic, anti-inflammation, antidiabetic, antiviral and antimicrobial effects as well as immunological and anticancer properties. From the edible mushrooms Helvella leucomelaena (Pers.) Nannf. "white-footed elf cup", belong to Helvellaceae Ascomycota's is a mushroom species. The aim of this study was to determine the antimicrobial activity of H. leucomelaena by using different concentrations of extracts obtained from solvents such as methanol, ethanol and hexane, and to determine the antioxidant activity of different concentrations of extract obtained using methanol solvent. Antimicrobial activity was determined by disc diffusion method. For the study, bacteria such as Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Escherichia coli ATCC25322 and Bacillus megaterium DSM32; Candida albicans FMC17 as fungi microorganisms were used. Antioxidant activity was determined according to 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical quenching capacity method. As a result, H. leucomelaena showed the highest inhibition zone against C. albicans (16 mm) at 750 µg concentration in ethanol extract. The scavenging effect of DPPH radical was observed to increase depend to increasing concentrations.
Mushrooms have been used by the public for many years due to the active containts they contain as well as being consumed as a nutritious food. It is known that mushrooms have antioxidant, antihypertensive, cholesterol-lowering, liver-protective, antifibriotic, anti-inflammation, antidiabetic, antiviral and antimicrobial effects as well as immunological and anticancer properties. From the edible mushrooms Helvella leucomelaena (Pers.) Nannf. "white-footed elf cup", belong to Helvellaceae Ascomycota's is a mushroom species. The aim of this study was to determine the antimicrobial activity of H. leucomelaena by using different concentrations of extracts obtained from solvents such as methanol, ethanol and hexane, and to determine the antioxidant activity of different concentrations of extract obtained using methanol solvent. Antimicrobial activity was determined by disc diffusion method. For the study, bacteria such as Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Escherichia coli ATCC25322 and Bacillus megaterium DSM32; Candida albicans FMC17 as fungi microorganisms were used. Antioxidant activity was determined according to 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical quenching capacity method. As a result, H. leucomelaena showed the highest inhibition zone against C. albicans (16 mm) at 750 µg concentration in ethanol extract. The scavenging effect of DPPH radical was observed to increase depend to increasing concentrations.
Açıklama
Anahtar Kelimeler
Helvella leucomelaena, Antimikrobiyal aktivite, Antioksidan aktivite, Antimicrobial activity, Antioxidant activity
Kaynak
Orman Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
2
Künye
İNCİ, Ş , KADIOĞLU DALKILIÇ, L , DALKILIÇ, S , KIRBAĞ, S . (2019). Helvella leucomelaena (Pers.) Nannf.’ın Antimikrobiyal ve Antioksidan Etkisi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi , 20 (2) , 249-253 . DOI: 10.17474/artvinofd.601528
