Isolation and characterization of native bacillus thuringiensis from Artvin, Turkey, and screening against hypantaria cunea (lepidoptera)

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dc.authorid0000-0001-8413-3190en_US
dc.authorid0000-0002-7757-6812en_US
dc.contributor.authorAlbayrak İskender, Nurcan
dc.contributor.authorSaral, Ayşegül
dc.date.accessioned2021-04-26T09:30:29Z
dc.date.available2021-04-26T09:30:29Z
dc.date.issued2017
dc.departmentAÇÜ, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.descriptionThis work was supported by the Artvin Coruh University Research Foundation (ACU-BAP 2014.F15.02.22)
dc.description.abstractThe soil in different regions may contain new B. thuringiensis species which may be toxic for the harmful insects. For this purpose, 5 native B. thuringiensis strains (Na1, Na2, Na5, Na7, Na25) were isolated from the soil of Artvin province in Turkey. The biochemical properties of the isolates (API 20 E and API 50 CH) were determined. The 16S rRNA gene sequence analyses, the polymerase chain reaction (ITS-PCR) of the 16S-23S intergenic transcribed spacer and the 16S rDNA based PCR-restriction fragment length polymorphism (RFLP) were performed. Then, cry gene content (cry 1, cry 2, cry 3, cry 4, and cry 9) of isolates were investigated. The conducted electron microscope research showed that the B. thuringiensis isolates carried four distinct crystal morphologies including rectangular, square, cubic, round and amorphous. The insecticidal effects of these isolates were investigated for 12 days in the 3-4th instar larvae of the Hypantria cunea (Lepidoptera). However, the isolates did not show any pathogenicity on the pest. In the later studies, it will be evaluated whether the isolates carry new crystal genes which are different from the control and whether they have entomopathogenic activity on different group of the insects.
dc.identifier.citationAlabayrak İskender, N., & Saral, A. (2017). Isolation and characterization of native bacillus thuringiensis from Artvin, Turkey, and screening against hypantaria cunea (lepidoptera).Fresenius Environmental Bulletin, 26(6), 4011- 4016en_US
dc.identifier.endpage4016en_US
dc.identifier.issue6en_US
dc.identifier.startpage4011en_US
dc.identifier.urihttps://hdl.handle.net/11494/3171
dc.identifier.volume26en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.institutionauthorAlbayrak İskender, Nurcan
dc.institutionauthorSaral, Ayşegül
dc.language.isoenen_US
dc.publisherParlar Scientific Publicationsen_US
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectSoilen_US
dc.subjectIsolationen_US
dc.subjectEntomopathogenic activityen_US
dc.subjectITS-PCRen_US
dc.subjectPCR-RFLPen_US
dc.titleIsolation and characterization of native bacillus thuringiensis from Artvin, Turkey, and screening against hypantaria cunea (lepidoptera)en_US
dc.typeArticle

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