Molecular docking analysis and determination of minimum inhibition concentration of VIM-38/R228S

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dc.contributor.authorDüzgün, Azer Ozad
dc.contributor.authorSaral Sarıyer, Ayşegül
dc.date.accessioned2025-07-07T11:27:02Z
dc.date.available2025-07-07T11:27:02Z
dc.date.issued2017
dc.departmentAÇÜ, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.description.abstractObjective: The aim of this study was to determine the effects of residues at 228th positions of VIM-38 on minimum inhibition concentration (MIC) and molecular docking analysis. Materials and methods: blaVIM-38 gene was cloned into expression vector pET100/D-TOPO. Then pET100/D-TOPO-VIM-38 was used to generate the R228S mutation by site-directed mutagenesis. The mutant was transformed into E. coli Dh5α. Changes in the activity of mutation R228S was determined by E-test. Molecular docking analysis of the binding affinity of meropenem and imipenem with VIM-38 and VIM-38 R228S was performed using AutoDock Vina_1_1_2. Results: According to E-test results, pET100/D-TOPO-VIM-38 and pET100/D-TOPO-VIM-38/ R228S have same MIC value for amoxicillin, ticarcillin/clavulanic acid, ampicillin and ticarcillin. It was determined decrease in pET100/D-TOPO-VIM-38/ R228S of MIC for aztreonam, amikacin and cefixine. There are 3-fold deference in MIC between pET100/D-TOPO-VIM-38 and pET100/D-TOPO-VIM-38/ R228S for cefoxitin. pET100/D-TOPO-VIM-38 has 0.5 µg/ml and pET100/D-TOPO-VIM-38/ R228S has 0.125 µg/m for imipenem, we observed 4-fold deference. Also, there is about 170-fold deference between pET100/D-TOPO-VIM-38 (16 µg/ml) and pET100/D-TOPO-VIM-38/ R228S (0.094 µg/ml) for meropenem. VIM-38 and VIM-38 R228S have very close to the negative low free energies of binding of the meropenem and imipenem. Conclusion: These results showed that substitution (R228S) slightly have effect on enzyme activity.
dc.identifier.endpage8937
dc.identifier.issn0970938X
dc.identifier.issue20
dc.identifier.startpage8933
dc.identifier.urihttps://hdl.handle.net/11494/5679
dc.identifier.volume28
dc.indekslendigikaynakScopus
dc.institutionauthorSaral Sarıyer, Ayşegül
dc.language.isoen
dc.publisherScientific Publishers of India
dc.relation.ispartofBiomedical Research (India)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDocking
dc.subjectMIC
dc.subjectMutation
dc.subjectVIM
dc.titleMolecular docking analysis and determination of minimum inhibition concentration of VIM-38/R228S
dc.typeArticle

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