Chemical characterization of water and ethanolic extracts of Turkish propolis byHPLC-DAD and GC-MS
Yükleniyor...
Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bellwether Publishing, Ltd.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study aims to determine the qualitative and quantitative contents of Turkish propolis collected from various regions of Turkey using high-performance liquid chromatography with the diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) in water and ethanolic extracts. In HPLC-DAD analyses, it was determined that water extract of Turkish propolis contains phenolic acids such as caffeic acid (204.00 µg/mL), trans-cinnamic, chlorogenic, and caffeoylquinic acids, responsible for its antioxidant activity; whereas, the ethanolic extract of Turkish propolis contains chrysin (641.33 µg/mL), caffeic acid phenethyl ester (630.67 µg/mL), pinocembrin (572.67 µg/mL), galangin (534.11 µg/mL), naringenin (372.39 µg/mL), and also kaempferol, trans-cinnamic acid, caffeic acid, myricetin, and quercetin. GC-MS analyses showed that ethanolic extract of propolis contains caffeic acid by Rtx-1 column and the water extract of propolis contains quinic acid and ferulic acid by Rtx-5ms column. Various sugar derivatives were detected by both columns in water and ethanolic extracts of Turkish propolis. HPLC-DAD can be considered as a more effective method than GC-MS for the chemical characterization of propolis. Water extract of Turkish propolis can be a good source of raw materials for various sectors, as it is both cheap and has less health risk than ethanolic extract, and is suitable for human use.
Açıklama
Anahtar Kelimeler
Ethanolic extract of propolis, GC-MS, HPLC-DAD, Polyphenols, Water extract of propolis
Kaynak
Journal of Liquid Chromatography and Related Technologies
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
Sayı
Künye
Tuğba Nigar Bozkuş , Orhan Değer & Ahmet Yaşar (2021): Chemicalcharacterization of water and ethanolic extracts of Turkish propolis by HPLC-DAD and GC-MS,Journal of Liquid Chromatography & Related Technologies, DOI: 10.1080/10826076.2021.1883648.
