Simultaneous determination of Nickel(II), Copper(II), and Cobalt(II) in environmental samples at trace levels by reversed-phase high-performance liquid chromatography using shiff base complexes
Yükleniyor...
Dosyalar
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pleiades Publishing
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
Abstract: A new method for the simultaneous determination of Ni(II), Cu(II), and Co(II) ions in environmental samples by reversed-phase liquid chromatography was developed. The developed method is based on the synthesis of the quadrivalent Schiff base 6,6'-((1E,1'E)-(ethane-1,2-diylbis(azaneylylidene))bis(methaneylylidene))bis(3-methoxyphenol) by the condensation reaction between 2-hydroxy-4-methoxybenzaldehyde and ethylenediamine. The resulting Schiff base was used as a chelating agent to convert the targeted ions into metal-ligand complexes within 1 min, which were extracted on an octadecylsilane (ODS) solid-phase extraction mini-column and then injected into a reversed-phase high-performance liquid chromatography system. Sufficient separation of the metal-ligand complexes was achieved on a GL Sciences Intersil ODS-3 column with a mobile phase composition of methanol-acetonitrile-water (30 : 30 : 40, v/v) within 6 min and detection by an ultraviolet detector at 254 nm. The limits of detection were found to be 0.45, 0.40, and 1.47 µg/L for Co2+, Cu2+, and Ni2+, respectively. Recoveries from water samples ranged from 97.4 to 103.6%, with relative standard deviation values between 0.4 and 1.7%. While the targeted metal ions were not detected in a sample from the remote upstream area of the Kabaca Stream (Artvin, Turkey), concentrations were found to be 116.4 µg/L for Cu2+ and 32.9 µg/L for Ni2+ in a sample taken from the Kabaca Stream near the industrial area (Artvin, Turkey). Co2+ was below the limit of detection in both cases. In addition, the developed method was also validated using the inductively coupled plasma optical emission spectrometry technique.
Açıklama
Anahtar Kelimeler
Diode array detector, HPLC, Schiff base, SPE, Trace elements
Kaynak
Journal of Analytical Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
80
Sayı
5
