Distribution of beta-lactamase genes among carbapenem-resistant klebsiella pneumoniae strains isolated from patients in Turkey

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dc.authorid0000-0003-0972-0461en_US
dc.authorid0000-0002-7757-6812en_US
dc.contributor.authorIraz, Meryem
dc.contributor.authorDüzgün, Azer Özad
dc.contributor.authorSandallı, Cemal
dc.contributor.authorDoymaz, Mehmet Ziya
dc.contributor.authorAkkoyunlu, Yasemin
dc.contributor.authorSaral, Ayşegül
dc.contributor.authorPeleg, Anton Y.
dc.contributor.authorÖzgümüş, Osman Birol
dc.contributor.authorBeris, Osman Birol
dc.contributor.authorBeris, Fatih Şaban
dc.contributor.authorKaraoglu, Hakan
dc.contributor.authorÇiçek, Ayşegül Çopur
dc.date.accessioned2021-09-23T06:34:41Z
dc.date.available2021-09-23T06:34:41Z
dc.date.issued2015
dc.departmentAÇÜ, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümüen_US
dc.descriptionThis work was supported by Recep Tayyip Erdogan University Research Fund Grants BAP-2013.102.03.12 and BAP-2013.102.03.13. A.Y.P was supported by an Australian National Health and Medical Research Council Career Development Fellowship (APP1047916).en_US
dc.description.abstractBackground: The emergence of carbapenem-resistant Klebsiella pneumoniae poses a serious problem to antibiotic management. We investigated the beta-lactamases in a group of carbapenem-resistant K. pneumoniae clinical isolates from Turkey. Methods: Thirty-seven strains of K. pneumoniae isolated from various clinical specimens were analyzed by antimicrobial susceptibility testing, PCR for the detection of p-lactamase genes, DNA sequencing, and repetitive extragenic palindronic (REP)- FOR analysis. Results: All 37 isolates were resistant to ampicillin, ampicillin/sulbactam, piperacillin, piperacillin/tazobactam, ceftazidime, cefoperazone/sulbactam, cefepime, imipenem, and meropenem. The lowest resistance rates were observed for colistin (2.7%), tigecycline (11%), and amikacin (19%). According to FOR and sequencing results, 98% (36/37) of strains carried at least one carbapenemase gene, with 32 (86%) carrying OXA-48 and 7 (19%) carrying NDM-1. No other carbapenemase genes were identified. All strains carried a CTX-M-2-like beta-lactamase, and some carried SHV- (97%), TEM- (9%), and CTX-M-1-like (62%) beta-lactamases. Sequence analysis of bla(TEM) genes identified a bla(TEM-166) with an amino acid change at position 53 (Arg53Gly) from bla(TEM-1b), the first report of a mutation in this region. REP-FOR analysis revealed that there were seven different clonal groups, and temporo-spatial links were identified within these groups. Conclusions: Combinations of beta-lactamases were found in all strains, with the most common being OXA-48, SHV, TEM, and CTX-M-type (76% of strains). We have reported, for the first time, a high prevalence of the NDM-1 (19%) carbapenemase in carbapenem-resistant K. pneumoniae from Turkey. These enzymes often co-exist with other beta-lactamases, such as TEM, SHV, and CTX-M beta-lactamases.
dc.identifier.citationIraz, M., Düzgün, A. Ö., Sandallı, C., Doymaz, M. Z., Akkoyunlu, Y., Saral, A., ... & Çiçek, A. Ç. (2015). Distribution of β-lactamase genes among carbapenem-resistant Klebsiella pneumoniae strains isolated from patients in Turkey. Annals of laboratory medicine, 35(6), 595-601.en_US
dc.identifier.doi10.3343/alm.2015.35.6.595
dc.identifier.endpage601en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage595en_US
dc.identifier.urihttps://hdl.handle.net/11494/3457
dc.identifier.volume35en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorSaral, Ayşegül
dc.language.isoenen_US
dc.publisherKorean Society for Laboratory Medicineen_US
dc.relation.ispartofAnnals of Laboratory Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbla(TEM-165)en_US
dc.subjectColistinen_US
dc.subjectK. pneumoniaeen_US
dc.subjectNDM-1en_US
dc.subjectOXA-48en_US
dc.titleDistribution of beta-lactamase genes among carbapenem-resistant klebsiella pneumoniae strains isolated from patients in Turkeyen_US
dc.typeArticle

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