Purification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activity

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dc.authoridTR130165en_US
dc.authorid0000-0001-5993-1668en_US
dc.contributor.authorCamadan, Yasemin
dc.contributor.authorÖzdemir, Hasan
dc.contributor.authorGülçin, İlhami
dc.date.accessioned2020-11-12T08:49:52Z
dc.date.available2020-11-12T08:49:52Z
dc.date.issued2016
dc.departmentAÇÜ, Sağlık Hizmetlerien_US
dc.description.abstractDihydropyrimidine dehydrogenase (DPD, E.C. 1.3.1.2) was purified from sheep liver with a yield of 16.7%, purification fold of 407.5 and specific activity of 0.705 EU/mg proteins. The purification procedure consisted of ammonium sulphate fractionation, DEAE ion exchange chromatography and 2',5'-ADP Sepharose-4B affinity chromatography. The molecular weight determined by SDS-PAGE and was found 111 kDa. Optimum pH, ionic strength temperature and stable pH were determined as 8.0, 0.9 mM, 50 degrees C and 6.0, respectively. The kinetic parameters (K-m and V-max) of the enzyme were determined with NADPH as 22.97 mu M and 0.17 EU/mL, respectively. The same parameters were determined with uracil as 17.46 mu M and 0.14 EU/mL, respectively. Additionally, in vitro inhibitory effects of some antidepressant drugs including escitalopram, fluoxetine, mirtazapine, haloperidol and some anaesthetic drugs including propofol and lidocaine were investigated against DPD. In addition, IC50 values for each active drug obtained for escitalopram, fluoxetine, mirtazapine, haloperidol, propofol and lidocaine were determined as 1736.11, 13.24, 86.65, 99.03, 0.21 and 15.07 mu M, respectively.
dc.identifier.citationCamadan, Y., Özdemir, H., & Gulcin, İ. (2016). Purification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1335-1341.en_US
dc.identifier.doi10.3109/14756366.2015.1132710
dc.identifier.endpage1341en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage1335en_US
dc.identifier.urihttps://hdl.handle.net/11494/2445
dc.identifier.volume31en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCamadan, Yasemin
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnaesthetic agenten_US
dc.subjectAntidepressant drugen_US
dc.subjectDihydropyrimidine dehydrogenaseen_US
dc.subjectEnzyme inhibitionen_US
dc.subjectEnzyme purificationen_US
dc.titlePurification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activityen_US
dc.typeArticle

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