Antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections in Turkey

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dc.authorid0000-0002-7757-6812en_US
dc.authorid0000-0002-1298-3687en_US
dc.contributor.authorÇopur Çiçek, Ayşegül
dc.contributor.authorÖzgümüş, Osman Birol
dc.contributor.authorSaral, Ayşegül
dc.contributor.authorSandallı, Cemal
dc.date.accessioned2020-11-13T07:53:24Z
dc.date.available2020-11-13T07:53:24Z
dc.date.issued2014
dc.departmentAÇÜ, Sağlık Bilimleri Fakültesien_US
dc.description.abstractWe aimed to observe antimiCrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Susceptibility to antimicrobials was tested using a disk diffusion method. The presence of integrons was examined using PCR with specific primers. Positive PCR results were confirmed by sequencing. A broth mating method was used for conjugation assays. Extragenic palindromic-FOR was performed using the oligonucleotide primer BOXA1R. Resistance frequency for ampicillin,trimethoprim/sulfamethoxazole, and tetracycline was determined as 50.6%, 33.5%, and 36.8% respectively. No strains were resistant to amikacin. Seventy isolates were positive for the intll gene, of which 49 carried gene cassettes. Eleven isolates were positive for the int12 gene, eight of swhich carried gene cassettes. Seven gene cassettes (dfrAl, dfrA5, dfrA7, dfrA17, aadAl, aadA5, and sat2) were predominantly harbored in integrons. We detected conjugative plasmids harboring integrons in two E. coli strains. Four strain clusters were yielded by BOX-FOR fingerprints showing that they were clonally related. No apparent relationship occurred among class 1 and 2 integron-carrying strains. We conclude that integrons are widespread in genetically variable E. coli strains and will continue to mediate dissemination of resistance genes in the community.We aimed to observe antimiCrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Susceptibility to antimicrobials was tested using a disk diffusion method. The presence of integrons was examined using PCR with specific primers. Positive PCR results were confirmed by sequencing. A broth mating method was used for conjugation assays. Extragenic palindromic-FOR was performed using the oligonucleotide primer BOXA1R. Resistance frequency for ampicillin, trimethoprim/sulfamethoxazole, and tetracycline was determined as 50.6%, 33.5%, and 36.8% respectively. No strains were resistant to amikacin. Seventy isolates were positive for the intll gene, of which 49 carried gene cassettes. Eleven isolates were positive for the int12 gene, eight of swhich carried gene cassettes. Seven gene cassettes (dfrAl, dfrA5, dfrA7, dfrA17, aadAl, aadA5, and sat2) were predominantly harbored in integrons. We detected conjugative plasmids harboring integrons in two E. coli strains. Four strain clusters were yielded by BOX-FOR fingerprints showing that they were clonally related. No apparent relationship occurred among class 1 and 2 integron-carrying strains. We conclude that integrons are widespread in genetically variable E. coli strains and will continue to mediate dissemination of resistance genes in the community.
dc.description.sponsorshipThis work was supported by Recep Tayyip Erdogan University Research Fund grants BAP-2012.106.01.11 and BAP-2011. 102.03.3.
dc.identifier.citationÇopur-Çiçek, A., Özgümüş, O. B., Saral, A., & Sandallı, C. (2014). Antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections in Turkey. Annals of Laboratory Medicine, 34(2), 139-144.en_US
dc.identifier.doi10.3343/alm.2014.34.2.139
dc.identifier.endpage144en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage139en_US
dc.identifier.urihttps://hdl.handle.net/11494/2453
dc.identifier.volume34en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSaral, Ayşegül
dc.language.isoenen_US
dc.publisherKorean Society for Laboratory Medicineen_US
dc.relation.ispartofAnnals of Laboratory Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntimicrobialen_US
dc.subjectResistanceen_US
dc.subjectIntegronsen_US
dc.subjectEscherichia colien_US
dc.subjectCommunity-acquired infectionsen_US
dc.titleAntimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections in Turkeyen_US
dc.typeArticle

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