Comprehensive phytochemical profiling and cytotoxic assessment of Caucasalia macrophylla coupled with in silico docking and miRNA (miR-19b, miR-20a, miR-126, miR-155, miR-200c)-Mediated gene regulation analysis

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dc.contributor.authorSarı, Zeynep Betül
dc.contributor.authorSarı, Muhammet Emin
dc.contributor.authorİncilay Torunoğlu, Emine
dc.contributor.authorDurmaz, Alper
dc.contributor.authorGümrükçüoğlu, Abidin
dc.contributor.authorAytar, Erdi Can
dc.date.accessioned2025-10-01T10:40:24Z
dc.date.available2025-10-01T10:40:24Z
dc.date.issued2025
dc.departmentAÇÜ, Rektörlük, Bilim Teknoloji Uygulama ve Araştırma Merkezi
dc.description.abstractThis study aimed to identify the chemical constituents of the methanol extract of Caucasalia macrophylla using GC-MS and HPLC-DAD techniques. The cytotoxic activity was determined by the MTT assay using human breast adenocarcinoma cells (MDA-MB-231) and hTERT-immortalized mammary epithelial cells (hTERT-HME1). The half-maximal inhibitory concentration (IC₅₀) for MDA-MB-231 cells was found to be 1.2 mg/ml. This concentration inhibited cell proliferation in a concentration-dependent manner. In addition, the extract was tested using a miRNA Isolation Kit to assess the mRNA expression profiles in both MDA-MB-231 and hTERT-HME1 cell lines. In cancer cells (MDA-MB-231), the expression of miR-126 was significantly upregulated 2.2-fold, while miR-155 and miR-200c were significantly downregulated 0.3-fold and 0.4-fold respectively. In healthy cells (hTERT-HME1), the expression levels of miR-19b, miR-155, and miR-200c were also significantly reduced. Further a comprehensive molecular docking analysis was conducted against phosphoinositide 3-kinase (PI3K). Among the tested compounds, rutin exhibited the strongest binding affinity with a binding energy of − 9.1 kcal/mol. This compound demonstrated potential anticancer effects by modulating PI3K activity. The findings suggest that modulation of signaling pathways by natural compounds may represent a promising approach for development of novel cancer therapies.
dc.identifier.doi10.1080/15287394.2025.2527653
dc.identifier.issn15287394
dc.identifier.pmid40600505
dc.identifier.scopus2-s2.0-105009727329
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://hdl.handle.net/11494/5934
dc.identifier.wosWOS:001521893400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorDurmaz, Alper
dc.institutionauthorGümrükçüoğlu, Abidin
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofJournal of Toxicology and Environmental Health - Part A: Current Issues
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.titleComprehensive phytochemical profiling and cytotoxic assessment of Caucasalia macrophylla coupled with in silico docking and miRNA (miR-19b, miR-20a, miR-126, miR-155, miR-200c)-Mediated gene regulation analysis
dc.typeArticle

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