Next-generation sequencing of plasmid carrying blaOXA-48 in Klebsiella pneumoniae from Turkey

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dc.authorid0000-0002-6301-611Xen_US
dc.authorid0000-0002-7757-6812en_US
dc.contributor.authorDüzgün, Azer Özad
dc.contributor.authorSaral, Ayşegül
dc.date.accessioned2020-11-04T06:13:17Z
dc.date.available2020-11-04T06:13:17Z
dc.date.issued2019
dc.departmentAÇÜ, Sağlık Bilimleri Fakültesien_US
dc.description.abstractA carbapenem-resistant Klebsiella pneumoniae strain was isolated in Turkey in 2012 and blaNDM-1 and blaOXA-48 genes were observed in this strain. The aim of this study was to investigate transferability of plasmid bearing blaOXA-48 in K. pneumoniae and to use whole-genome sequencing in order to understand the genetic context of plasmid. K. pneumoniae strain was used as donor in conjugation experiments. Antibiotic susceptibility profile of selected transconjugant was determined. Plasmid was isolated from transconjugant colony and was named as pKPT. Complete sequencing of the pKPT was conducted using a next-generation sequencing. Annotation of the contigs was performed using the Geneious R9, followed by finding open reading frames (ORFs) with selected web-based tools. BLAST analysis was performed at the NCBI BLAST server to determine genes showing more than 90% similarity with these ORFs. Results of antibiotic susceptibility test showed that transconjugant colony was resistant to ampicillin/ sulbactam, piperacillin, and piperacillin/tazobactam. The pKPT plasmid had a length of 45,217 bp and an average G + C content of 49%. Blast analysis revealed that pKPT was included in the IncL/M incompatibility group. The pKPT was found to contain blaOXA-48 within Tn1999.2 transposon without any other antibiotic resistance gene.
dc.description.sponsorshipGumushane University: BAP-17 F5119.02.01en_US
dc.identifier.citationDüzgün, A. Ö., & Saral, A. (2019). Next-generation sequencing of plasmid carrying bla OXA-48 in Klebsiella pneumoniae from Turkey. Acta Microbiologica et Immunologica Hungarica, 66(2), 261-272.en_US
dc.identifier.doi10.1556/030.66.2019.010
dc.identifier.endpage272en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3
dc.identifier.startpage261en_US
dc.identifier.urihttps://hdl.handle.net/11494/2404
dc.identifier.volume66en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSaral, Ayşegül
dc.language.isoenen_US
dc.publisherAkademia Kiado ZRTen_US
dc.relation.ispartofActa Microbiologica et Immunologica Hungarica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectPlasmiden_US
dc.subjectBlaOXA-48en_US
dc.subjectNext-generation sequencingen_US
dc.subjectConjugationen_US
dc.titleNext-generation sequencing of plasmid carrying blaOXA-48 in Klebsiella pneumoniae from Turkeyen_US
dc.typeArticle

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