Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities
| dc.authorid | 0000-0002-0505-3190 | en_US |
| dc.authorid | 0000-0002-7149-7427 | en_US |
| dc.authorid | 0000-0002-9550-9111 | en_US |
| dc.authorid | 0000-0003-0064-0318 | en_US |
| dc.authorid | 0000-0002-7946-6545 | en_US |
| dc.contributor.author | Erenler, Ramazan | |
| dc.contributor.author | Meral, Bilal | |
| dc.contributor.author | Şen, Özkan | |
| dc.contributor.author | Elmastaş, Mahfuz | |
| dc.contributor.author | Aydın, Ali | |
| dc.contributor.author | Eminağaoğlu, Özgür | |
| dc.contributor.author | Topçu, Gulacti | |
| dc.date.accessioned | 2020-11-06T10:36:17Z | |
| dc.date.available | 2020-11-06T10:36:17Z | |
| dc.date.issued | 2017 | |
| dc.department | AÇÜ, Orman Fakültesi | en_US |
| dc.description.abstract | Context: Origanum (Lamiaceae) has been used in food and pharmaceutical industries. Objective: Isolation and identification of bioactive compounds from Origanum rotundifolium Boiss. and investigation of their antiproliferative and antioxidant activities. Materials and methods: The aerial part of O. rotundifolium was dried and powdered (1.0 kg ±2.0 g) then extracted with hexane, ethyl acetate, methanol and water. Solvent (3 1 L) was used for each extraction for a week at room temperature. The aqueous extract was partitioned with ethyl acetate (3 1 L) to yield the water/EtOAc extract subjected to chromatography to isolate the active compounds. The structures of isolated compounds were elucidated by 1 D, 2 D NMR and LC-TOF/MS. Results: Apigenin (1), ferulic acid (2), vitexin (3), caprolactam (4), rosmarinic acid (5), and globoidnan A (6) were isolated and identified. Globoidnan A (6), vitexin (3), and rosmarinic acid (5) revealed the excellent DPPH scavenging effect with IC50 values of 22.4, 31.4, 47.2 lM, respectively. Vitexin (3) (IC50 3.6), globoidnan A (6) (IC50 4.6), apigenin (1) (IC50 8.9) and ferulic acid (2) exhibited more ABTS? activity than standard Trolox (IC50 13.8 lg/mL). Vitexin (3) revealed the most antiproliferative activity against HeLa, HT29, C6 and Vero cells lines with IC50 values of 35.6, 32.5, 41.6, 46.7 (lM), respectively. Discussion and conclusion: Globoidnan A (6) has the most antioxidant effects on all assays. This has to do with the chemical structure of the compound bearing the acidic protons. Vitexin (3) could be a promising anticancer agent. | |
| dc.identifier.citation | Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55(1), 1646-1653. | en_US |
| dc.identifier.doi | 10.1080/13880209.2017.1310906 | |
| dc.identifier.endpage | 1653 | en_US |
| dc.identifier.issue | 1 | en_US |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.startpage | 1646 | en_US |
| dc.identifier.uri | https://hdl.handle.net/11494/2422 | |
| dc.identifier.volume | 55 | en_US |
| dc.identifier.wosquality | N/A | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.institutionauthor | Eminağaoğlu, Özgür | |
| dc.language.iso | en | en_US |
| dc.publisher | Taylor & Francis Inc | en_US |
| dc.relation.ispartof | Pharmaceutical Biology | |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.relation.tubitak | 113Z195 | |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Secondary metabolites | en_US |
| dc.subject | Isolation | en_US |
| dc.subject | Chromatography | en_US |
| dc.subject | Spectroscopy | en_US |
| dc.title | Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities | en_US |
| dc.type | Article |
