High-fructose consumption suppresses insulin signaling pathway accompanied by activation of macrophage and apoptotic markers in rat testis

Basit Öğe Kaydı
dc.authorid0000–0003-0090–7369
dc.authorid0000–0002-3267–2886
dc.authorid0000–0002-5165–5854
dc.authorid0000–0002-5432–0304
dc.contributor.authorYıldırım, Onur Gökhan
dc.contributor.authorGüney, Ceren
dc.contributor.authorAlcigir, Mehmet Eray
dc.contributor.authorAkar, Fatma
dc.date.accessioned2025-07-17T06:23:56Z
dc.date.available2025-07-17T06:23:56Z
dc.date.issued2023
dc.departmentAÇÜ, Sağlık Hizmetleri Meslek Yüksekokulu, Eczane Hizmetleri Bölümü
dc.description.abstractDietary high-fructose may cause metabolic disturbances; however, its effect on the reproductive system is little understood. The insulin signaling pathway is critical in testicular development, maintenance of microcirculation and spermatogenesis. Therefore, in this study, we aimed to investigate the impact of dietary high-fructose on insulin signaling pathway as well as macrophage and apoptotic markers in testicular tissue of rats. Fructose was administered to male Wistar rats as a 20% solution in drinking water for fifteen-week. Gene expression of ir-β, irs-1, irs-2, pi3k, akt, mtor, and enos in the testicular samples was determined by real-time PCR. Protein expression of IR, IRS-1, IRS-2, PI3K, Akt, phospho-Akt (p-Akt), mTOR, eNOS, phospho-eNOS (p-eNOS), and GLUT5 was established by analysis of Western Blot. Testicular expression of occludin, CD163, CD68, caspase-8, and caspase-3 was analyzed by using immunohistochemical assay. Testicular level of fructose was measured by colorimetric method. Dietary high-fructose decreased mRNA expressions of irs-1, irs-2, pi3k, and mtor in the testicular tissue of rats. Also, this dietary intervention impaired protein expressions of IR, IRS-1, IRS-2, PI3K, p-Akt, mTOR, eNOS, and p-eNOS as well as p-Akt/Akt and p-eNOS/eNOS ratios in the testis of rats. However, a high-fructose diet increased the expression of CD163, CD68, caspase-8 and caspase-3, but decreased that of occludin, in the testicular tissue of rats. The high-fructose consumption in rats suppresses testicular insulin signaling but activates macrophages-related factors and apoptotic markers. These changes induced by dietary fructose could be related to male reproductive dysfunction.
dc.identifier.doi10.1016/j.repbio.2023.100815
dc.identifier.issn1642431X
dc.identifier.issue4
dc.identifier.scopuss2.0-85174030212
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://hdl.handle.net/11494/5781
dc.identifier.volume23
dc.indekslendigikaynakScopus
dc.institutionauthorYıldırım, Onur Gökhan
dc.institutionauthorid0000–0003-0090–7369
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofReproductive Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectApoptosis
dc.subjectDietary fructose
dc.subjectInsulin signaling pathway
dc.subjectMacrophages
dc.subjectRat testis
dc.titleHigh-fructose consumption suppresses insulin signaling pathway accompanied by activation of macrophage and apoptotic markers in rat testis
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
5781.pdf
Boyut:
10.59 MB
Biçim:
Adobe Portable Document Format
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Sağlık Hizmetleri Meslek Yüksekokulu Yayın Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu