Effects of Globularia cordifolia on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies

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dc.contributor.authorTorunoglu, Emine İncilay
dc.contributor.authorAytar, Erdi Can
dc.contributor.authorAydın, Betül
dc.contributor.authorDurmaz, Alper
dc.contributor.authorBalpınar, Özge
dc.contributor.authorErtuğrul, Muhammed Sait
dc.contributor.authorYarım, Gül Fatma
dc.date.accessioned2025-03-12T13:05:13Z
dc.date.available2025-03-12T13:05:13Z
dc.date.issued2024
dc.departmentAÇÜ
dc.description.abstractBACKGROUND: The present study aimed to comprehensively evaluate the anticancer, anti-inflammatory, and antioxidant properties of Globularia cordifolia L. samples. The plant material was collected and extracted using the maceration method. Antioxidant activities were assessed through DPPH (i.e. 2,2-diphenyl-1-picrylhydrazyl) and iron chelation assays, whereas phenolic and flavonoid contents were measured using spectrophotometric methods. Gas chromatography-mass spectrometry analysis was performed to identify biologically active compounds. The cytotoxic effect on colon cancer cells was evaluated using MTT [i.e. 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] assays, and inflammatory cytokine levels were measured through an enzyme-linked immunosorbent assay. Molecular docking studies investigated the interactions of bioactive compounds with the 4UYA protein, and ADME (i.e. absorption, distribution, metabolism, excretion and toxicity) modeling predicted their toxicity. RESULTS: The study revealed that the extract exhibited strong antioxidant activity with a median 50% inhibitory concentration (IC50) value of 39.48 ± 2.15 μg mL−1 in the DPPH assay and an effective iron chelation capacity with an IC50 value of 4.28 ± 0.19 mg mL−1. The total phenolic content was 662.51 ± 20.95 mg gallic acid equivalents g−1, and the flavonoid content was 114.99 ± 0.51 mg quercetin equivalents g−1, demonstrating the extract's richness in phenolic and flavonoid compounds. Significant antiproliferative effects were observed in colon cancer cells at a dose of 250 μg mL−1. Inflammatory cytokine analysis indicated a reduction in pro-inflammatory responses. Molecular docking studies showed strong binding affinities between the bioactive compounds and the 4UYA protein, suggesting the mechanisms of action. CONCLUSION: The findings indicate that G. cordifolia extract exhibits strong antioxidant, anti-inflammatory and antiproliferative properties, making it a promising biomolecular agent for colon cancer therapy. The high phenolic and flavonoid contents, combined with the bioactive compounds' interactions with key molecular targets, underscore its potential therapeutic applications.
dc.identifier.doi10.1002/jsfa.14100
dc.identifier.issn00225142
dc.identifier.pmid39731258
dc.identifier.scopus2-s2.0-85213495258
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/11494/5439
dc.identifier.wosWOS:001385092800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorDurmaz, Alper
dc.language.isoen
dc.publisherJohn Wiley and Sons Ltd
dc.relation.ispartofJournal of the Science of Food and Agriculture
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntioxidant activity
dc.subjectAntiproliferative effects
dc.subjectColon cancer
dc.subjectGlobularia cordifolia
dc.subjectMolecular docking
dc.titleEffects of Globularia cordifolia on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies
dc.typeArticle

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