The protein-protein interactions between Amsacta moorei entomopoxvirus (AMEV) protein kinases (PKs) and all viral proteins

Basit Öğe Kaydı
dc.authoridTR122862en_US
dc.contributor.authorDanışmazğlu, Mehtap
dc.contributor.authorNalçacıoğlu, Kadriye
dc.contributor.authorMuratoğlu, Hacer
dc.contributor.authorDemirbağ, Zihni
dc.date.accessioned2020-11-03T08:18:44Z
dc.date.available2020-11-03T08:18:44Z
dc.date.issued2018
dc.departmentAÇÜ, Sağlık Hizmetlerien_US
dc.description.abstractEntomopoxviruses are an important group of viruses infecting only insects. They belong to Poxviridae which infect both invertebrates and vertebrates, including humans. Protein kinases are known to have roles at virus morphogenesis, host selectivity, the regulation of cell division and apoptosis in some vertebrate poxviruses. In this study, 2 protein kinases (PKs) (AMV153 and AMV197) of Amsacta moorei entomopoxvirus (AMEV) were investigated for the interactions among 230 viral proteins using yeast two-hybrid system (Y2H). For this purpose, two protein kinases and 230 viral genes were cloned into the bait and prey vectors, respectively. Bait vectors were introduced into Saccharomyces cerevisiae AH109. Expression of the bait genes were confirmed by western blot analysis. Both yeast strains of bait were transformed individually with each prey clone and grown on a selective medium (minimal synthetic defined) to determine the protein–protein interactions between bait and prey proteins. Transformations identified totally 16 interactions among AMEV protein kinases and all viral proteins of which 5 belong to AMV153 and 11 belong to AMV197. One of the five interactions detected for AMV153 protein kinase is self-association. Its other four interactions are with two virus entry complex proteins (AMV035 and AMV083), a membrane protein (AMV165) and a subunit of RNA polymerase (AMV230). The other protein kinase, AMV197, interacted with two virus entry complex proteins (AMV035 and AMV083) as AMV153, a caspase-2 enzyme (AMV063), a Holliday junction resolvase (AMV162), a membrane protein (AMV165), a subunit of RNA polymerase (AMV230) and five other hypothetical proteins (AMV026, AMV040, AMV062, AMV069, AMV120) encoded by AMEV genome. Glutathione S-transferase (GST) pull-down assay was used to confirm all interactions described by Y2H analysis. In addition, the theoretical structures of the two of 16 interactions were interpreted by docking analysis. Consistent with Y2H and pull down assays, docking analysis also showed the interactions of AMV063 with AMV153 and AMV197. Detected interactions of the AMEV viral proteins with viral protein kinases could lead to the understanding of the regulation of the viral activities of interacted viral proteins.
dc.identifier.citationDanışmazoğlu, M., Nalcacıoğlu, R., Muratoğlu, H., & Demirbağ, Z. (2018). The protein-protein interactions between Amsacta moorei entomopoxvirus (AMEV) protein kinases (PKs) and all viral proteins. Virus research, 248, 31-38. Doi: 10.1016/j.virusres.2018.02.007en_US
dc.identifier.doi10.1016/j.virusres.2018.02.007
dc.identifier.endpage38en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage31en_US
dc.identifier.urihttps://hdl.handle.net/11494/2401
dc.identifier.volume248en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDanışmazğlu, Mehtap
dc.language.isoenen_US
dc.publisherElsevier Science BVen_US
dc.relation.ispartofVirus Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak113Z081
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmsacta moorei entomopoxvirusen_US
dc.subjectSerine/threonine protein kinasesen_US
dc.subjectYeast two-hybrid systemen_US
dc.subjectProtein-protein interactionsen_US
dc.subjectAMV153en_US
dc.subjectAMV197en_US
dc.titleThe protein-protein interactions between Amsacta moorei entomopoxvirus (AMEV) protein kinases (PKs) and all viral proteinsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
mehtap.danismazoglu.pdf
Boyut:
576.64 KB
Biçim:
Adobe Portable Document Format
Açıklama:
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Sağlık Hizmetleri Meslek Yüksekokulu Yayın Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu