The ameliorative effect of Cetraria islandica against diabetes-induced genetic and oxidative damage in human blood

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dc.contributor.authorÇolak, Suat
dc.contributor.authorGeyikoğlu, Fatime
dc.contributor.authorTürkez, Hasan
dc.contributor.authorBakır, Tülay Özhan
dc.contributor.authorAslan, Ali
dc.date.accessioned2020-11-13T13:25:12Z
dc.date.available2020-11-13T13:25:12Z
dc.date.issued2013
dc.departmentAÇÜ, Rektörlüken_US
dc.description.abstractContext: The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions. Objective: The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects. Materials and methods: Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 mu g mL(-1)) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes. Results: There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p>0.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL(-1) lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates. Discussion: In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.
dc.description.sponsorshipThis investigation was supported by Ataturk University (BAP-2004/172 and 2008/76).en_US
dc.identifier.citationÇolak, S., Geyikoglu, F., Türkez, H., Bakır, T. Ö., & Aslan, A. (2013). The ameliorative effect of Cetraria islandica against diabetes-induced genetic and oxidative damage in human blood. Pharmaceutical Biology, 51(12), 1531-1537.en_US
dc.identifier.doi10.3109/13880209.2013.801994
dc.identifier.endpage1537en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage1531en_US
dc.identifier.urihttps://hdl.handle.net/11494/2458
dc.identifier.volume51en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÇolak, Suat
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPharmaceutical Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiabetes mellitusen_US
dc.subjectGenotoxicityen_US
dc.subjectHuman bloodculturesen_US
dc.subjectLichenen_US
dc.subjectOxidative stressen_US
dc.titleThe ameliorative effect of Cetraria islandica against diabetes-induced genetic and oxidative damage in human blooden_US
dc.typeArticle

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