Combined anticancer effects of Chamaecytisus hirsutus extract and photodynamic therapy in Gastric cancer cells

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dc.contributor.authorÇalışkan, Metin
dc.contributor.authorÇalışkan, Serçin Özlem
dc.contributor.authorİncilay Torunoğlu, Emine
dc.contributor.authorDurmaz, Alper
dc.contributor.authorDüzgün, Zekeriya
dc.contributor.authorAytar, Erdi Can
dc.date.accessioned2025-06-19T10:33:43Z
dc.date.available2025-06-19T10:33:43Z
dc.date.issued2025
dc.departmentAÇÜ
dc.description.abstractThe particular plant found in Türkiye, Chamaecytisus hirsutus;(Fabaceae), has no studies examining its biological effects. Thus, the present study aimed to investigate the phytochemical composition, chemical profile, antioxidant activity, xanthine oxidase inhibition, anticancer effects, and photodynamic therapy potential of C. hirsutus methanol extract. Gas chromatography-mass spectrometry (GC-MS) analysis identified important compounds such as laminitol (28.74%), quinolinic acid (20.23%), and 2-(1,3-Dioxan-2-yl) ethanol (17.27%). The IC50 value of the extract in the DPPH assay was 378.77 ± 4.70 mg/mL, demonstrating significant antioxidant activity. The xanthine oxidase inhibition was found to be 45.75 ± 2.59%, indicating significant free radical scavenging activity. The total phenolic, flavonoid, flavanols, and tannin contents were determined to be 35.14 ± 2.60 mg GAE/g extract, 12.90 ± 0.27 mg QE/g extract, 20.67 ± 0.81 mg QE/g extract, and 2.49 ± 0.12 mg GAE/g extract, respectively. The extract reduced the viability of MKN28 cancer cells by approximately 85% at a concentration of 0.4 mg/mL, demonstrating significant anticancer activity. Combination therapy with photodynamic therapy further enhanced the cytotoxic effect. Additionally, the binding affinities of these compounds with the 1GUX target protein were evaluated using molecular docking, and 4-Methyl-2-pentadecyl-1,3-dioxolane and Laminitol were determined as the strongest inhibitors with binding energies of −6.9 kcal/mol and −6.1 kcal/mol, respectively. Molecular dynamics simulations confirmed the stability of these interactions, with 4-Methyl-2-pentadecyl-1,3-dioxolane emerging as the compound with the strongest binding affinity. These findings suggest that C. hirsutus extract has the potential to be a powerful anticancer agent when used in photodynamic therapy.
dc.identifier.doi10.1080/15287394.2025.2508839
dc.identifier.issn15287394
dc.identifier.pmid40401701
dc.identifier.scopus2-s2.0-105005867340
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://hdl.handle.net/11494/5535
dc.identifier.wosWOS:001493864200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorDurmaz, Alper
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofJournal of Toxicology and Environmental Health - Part A: Current Issues
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.titleCombined anticancer effects of Chamaecytisus hirsutus extract and photodynamic therapy in Gastric cancer cells
dc.typeArticle

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